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Unformatted text preview: 3. RNA is removed and replaced by RNA polymerase I 4. helicase uncoils strands, primase puts down RNA primer, DNA polymerase III builds on chain on leading RNA strand (from 3 of DNA on), builds Okazaki fragments on lagging strand (5 on) Okazaki fragments: 1. RNA primase forms RNA primer 2. Primase released 3. DNA polymerase III synthesizes strand until it meets the previous one 4. DNA polymerase I replaces RNA primer 5. DNA ligase stitches the pieces together DNA polymerase III has an error rate of about 1 in 10,000 base pairs Final error rate is ~ 1 in a billion Poly III can do approx 1000 bp/second in E. coli slower in eukaryotes Rotates at about 3000 revolutions per minutes Telomeres Take a sequence like GGGATT its repeated around 2500 times in humans Telomerase: preserves length of chromosomes on repeated sequences...
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- Spring '08