EnzymesLabReport - Williams 1 MylaSha Williams Professor...

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Williams 1 Myla’Sha Williams Professor Randolph BSC1010C 26 February 2016 Enzyme Lab Report Introduction: Enzymes are the proteins in organisms that help chemical reactions. They are made up of amino acids, which are highly impressionable to differences in pH or temperature due to the fact that the shape of amino acids is dependent on their bonds. These changes may cause the shape of the enzyme itself to become changed as well, making the enzyme denatured, or non-functional. A catalase enzyme is an enzyme found in most living organisms. The one used in this lab is was from the liver. It is needed because it changes hydrogen peroxide to water and oxygen. Materials and Methods: In this lab, we used three different experiment sets to test the effects differences in concentration, temperature, and pH have on the activity of the catalase enzyme. For all three experiment sets, we created a setup to measure the reaction times of the effects. The setup included an inverted eudiometer, which was completely filled with water in order to measure when each reaction generated 9ml of oxygen. The eudiometer was placed into a beaker half filled with water, which we connected to the reaction flask, where the enzyme generated the oxygen, by using a hook on a rubber hose connected to the black stopper we put in the reaction flask.
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