Exam II - Fall 2002 - MCB 316 Exam 2 Sample Essay Questions...

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MCB 316 Exam 2 Sample Essay Questions : 1. If a single strand of a double-stranded helix has the sequence 5'-GTTTAACGTAT-3', draw out the complementary strand and indicate strand polarity. If the helix was in Bform, how many turns (360 degrees) would the helix make? ( Note that the book cites 10 bp per turn but the actual number is 10.5 bp per turn. ) 2. Compare and contrast eukaryotic replication versus prokaryotic replication. 3. Eukaryotes have three principle means of RNA processing. List each process and identify its biological significance. 4. In what way are the mutagens nitrous acid and bromouracil similar? In what ways are they different? 5. Compare and contrast differences in translational inititation in prokaryotes verses eukaryotes. 6. If the P element of Drosophila contained no introns, would this affect the viability of P element carriers? If so, How? Sample Multiple Choice Problems : 1. Experiments subsequent to Griffith's 1928 experiment showed that: a. the transformation principle could be carried out in vitro . b. the conversion of Type IIR to Type IIIS was due to mutation. c. the process of heat-killing cells was not entirely effective. d. DNAse treatment eliminates all transforming activity. e. a and d f. all of the above
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