BILD 10 - 11.3

BILD 10 - 11.3 - BILD 10: 11/03 Notes: Applications to...

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BILD 10: 11/03 Notes: Applications to Genetic Engineering - Reduce production cost of specific proteins - Produce improved crops and livestock - Forensic and paternity cases - Determine function of a gene - Determine cause of disease - Design improved drugs - Gene therapy Prof. Green is old Prof. Green is wearing a shirt that says Caffeine How do restriction enzymes work? - Restriction enzymes cut palindromic sequences - i.e ===GAATTC==========GAATTC=== ===CTTAAG==========CTTAAG=== - Restriction enzyme cuts the DNA into fragments. - MADAM - is a palindrome. - Palindrome is a something that can be spelled the same backwards and forwards. * Note - I did not know that. - Addition of a DNA fragment from another source; the two fragments stick together by base pairing. - AGC CGA Then fill in the base pairs. - AGC G C T T C G CGA - Restriction enzymes have two active sites -- one to cut Watson strand, and one to cut the Crick strand. - Palindromic sequences are usually 6 pairs - After the enzymes cut the DNA, it begins to fall apart. - Why is that important? Genetic engineers recognize this because the DNA strands are palindromic and sticky therefore, allowing it to be joined together from two different sources - DNA ligase seals the strands. - The new strand is called Recombinant DNA
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This note was uploaded on 04/22/2008 for the course BILD 10 taught by Professor Banks during the Fall '08 term at UCSD.

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BILD 10 - 11.3 - BILD 10: 11/03 Notes: Applications to...

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