Bio Lab report 2-ELISA

Bio Lab report 2-ELISA - Using the Elisa Technique and...

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Using the Elisa Technique and Blood Typing to describe antibody-antigen reactions Introduction This experiment aims to investigate the hypothesis that the absorbance read from the spectrophotometer will decrease as the dilution increases from the 1 st well to the 11 th .The ELISA and blood typing experiment is worth investigating because it can be performed to evaluate either the presence of antigen or the presence of antibody in a sample, it is a useful tool both for determining serum antibody concentrations (such as HIV) and also for detecting the presence of antigen. (Wikipedia, 2007). The ELISA (Enzyme-linked immunosorbent assay) technique can be used to diagnose diseases by 2 methods—direct and indirect. The direct ELISA method detects antigens while the indirect method (which is the technique we used in our experiment) detects antibodies. It does this by agglutination reactions that occur when antibodies and antigens interact. ELISA uses enzyme labeled antibodies to detect an antigen or antibody (Lum, 2006). This diagram shows the steps taken to prepare an indirect ELISA technique:
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A patient’s blood type can be detected by its antibody-antigen interaction. When foreign antigens are found, red blood cells either burst or clump together by agglutination (Lum, 2006). If agglutination in your blood occurs, this indicates the presence of one of the ABO blood types. Type A blood contains antigen A and anti-B antibodies, while Type B is the opposite of this. Type AB (also known as the universal receiver) contains both antigens A and B and neither anti-A or B antibodies. Type O (also known as the universal donor) contains neither A or B antigens but both Anti-A and B antibodies. This can be applied by testing a patient’s blood sample. If the blood agglutinates after the addition of anti A serum, this shows the person has Type A blood. If no agglutination happens in after the addition of anti A or anti B serum-this person has Type O blood. We performed this experiment by using the indirect ELISA technique by diluting the patient’s serum from the first well to the last. This is the reason why I predict that the absorbance will decrease, since the color of the enzyme substrate will be progressively lighter. Materials and Methods
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Bio Lab report 2-ELISA - Using the Elisa Technique and...

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