Drop-Out Media Plating and Plasmid Transformation

Drop-Out Media Plating and Plasmid Transformation -...

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Auxotrophs: mutants which have altered nutritional requirements due to  the mutation of one or more genes.  They are derived from wild type or  prototype yeast strains. Saccharomyces Cerevisiae: The availability of Saccharomyces  cerevisiae yeast strains with multiple auxotrophic markers allows the  stable introduction and selection of more than one yeast shuttle vector  containing marker genes that complement the auxotrophic markers  (Source 1) needs to be summarized. Need source 2 info.  Need source 2.
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Week 1: Hypothesis: We predict that our strain of yeast will grow on the YPD  (positive control) plate and any plate where the yeast is able to reproduce  with an environment that lacks a specific amino acid/nucleotide that the  yeast is already providing. Week 2: Hypothesis: We predict when a yeast strain auxotrophic for HIS is  placed in an environment lacking HIS, but given a plasmid that carries the 
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Drop-Out Media Plating and Plasmid Transformation -...

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