Genetic Mutation of Yeast (show)

Genetic Mutation of Yeast (show) - Genetic Mutation of...

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Genetic Mutation of Yeast Genetic Mutation of Yeast Cody Burrum, Allison Graham, Liz Jeans, Amber Owens
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Introduction Auxotrophs: mutants which have altered nutritional  requirements due to the mutation of one or more genes.   They are derived from wild type or prototype yeast strains. Saccharomyces Cerevisiae: Since Saccharomyces  cerevisiae strains have many auxotrophic  markers, they  are abundant. It allows a scientist to work with the yeast by  selecting set portions of the gene sequence in order to  compliment an auxotrophic strand (Frazer LN, O’Keefe  RT).  www.toastmonster.com
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Hypotheses Week 1: We predict that our strain of yeast will grow on the  YPD (positive control) plate and any plate where the yeast  is able to reproduce with an environment that lacks a  specific amino acid/nucleotide that the yeast is already  providing. Week 2: We predict when a yeast strain auxotrophic for  HIS is placed in an environment lacking HIS, but given a  plasmid that carries the HIS, it will grow. Null Hypothesis: We predict that the yeast will not grow on  any plate because no plates will provide the correct  environment. 
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Methods 6 agar plates Positive (YPD), Negative (HTLU), Leucine, Uracil, Histidine, Tryptophan Wild Type? Auxotroph?
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Genetic Mutation of Yeast (show) - Genetic Mutation of...

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