Genetic Mutation show 2

Genetic Mutation show 2 - Genetic Mutation of Yeast Cody...

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Genetic Mutation of Genetic Mutation of Yeast Yeast Cody Burrum, Allison Graham, Liz Jeans, Amber Owens
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Introduction Auxotrophs: mutants which have altered nutritional requirements due to the mutation of one or more genes. They are derived from wild type or prototype yeast strains. Saccharomyces cerevisiae strains have many auxotrophic markers. These markers allow for many options and possibilities for manipulation. This allows a scientist to work with the yeast by selecting set portions of the gene sequence in order to compliment an auxotrophic strand (Frazer and O’Keefe 2007). www.toastmonster.com
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Hypotheses Week 1: We predict that our strain of yeast will grow on the YPD (positive control) plate and any plate where the yeast is able to reproduce in an environment that lacks a specific amino acid/nucleotide that the yeast is already providing. Week 2: We predict when a yeast strain auxotrophic for HIS is placed in an environment lacking HIS, but given a plasmid that carries the correct DNA sequence to code for HIS, it will grow. Null Hypothesis: We predict that the yeast will not grow on any plate because no plates will provide the correct environment.
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Methods 6 agar plates Positive (YPD), Negative (HTLU), Leucine, Uracil, Histidine, Tryptophan To Rescue Histidine…
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Genetic Mutation show 2 - Genetic Mutation of Yeast Cody...

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