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Unit 6 Restriction Digest Lab .pdf - Maham Malik March...

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Maham MalikMarch 21st, 2022Restriction Digest and Analysis of Lambda Phage DNA LabTo determine the DNA fragment sizes in this investigation, we measured the distance eachfragment of DNA was from the bands in mm. The enzyme cuts the DNA at different locationsand the DNA fragments are stained at different distances from each other and the bandsdepending on their relative sizes. An example of this recorded in our data table is the DNAfragments that appeared on the gel and were cut by Hindii restriction were measured to be 8mm,10mm, and 13mm. The varying distance of fragments was due to the number of estimated basepairs.In this experiment the gel lanes that served as the control were the DNA marker. There was oneband of DNA marker that was formed on the gel. The DNA sample tubes included Lambda, PstI,EcoRI, HindIII restriction digests and 1, 2, 2 and 3 restriction fragments formed for each
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Term
Fall
Professor
Molly Reilly
Tags
molecular biology, DNA, DNA fragment, Restriction enzyme, Lambda Phage DNA Lab

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