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Bacterial Transformation Lab Report.pdf - Maham Malik March...

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Maham MalikMarch 7th, 2022Bacterial Transformation Lab ReportResults Table-pGLO/LB-pGLO/LB/amp+pGLO/LB/amp+pGLO/LB/amp/aragrowth?nonononoglow?nonononoData AnalysisOur transformation process did not work and we could tell by the fact that little to no coloniesgrew on any of the plates. Especially the -pGLO/LB plate since no ampicillin was added to theplate so bacterial growth should have been guaranteed. A source of error that could haveprevented the transformation from working properly could have been that in step 11, we didnot use the proper motion to spread the suspensions evenly around the surface of the agarplate and did not skate the sterile loop back and forth across the plate. Rather we scratchedagar with the +pGLO and -pGLO broths in a single line. Not sliding across the plate with thebroths did not allow enough bacteria to be transformed or grow.DiscussionA bacterial transformation is when you take one bacterium, for example, e. choli, and transformit into another by passing its DNA along.A plasmid is a small circular DNA strand in the bacteriathat can be genetically engineered to transport genes from one bacterium to another. Plasmidswere also used in this particular lab to transform the bacteria. On the pGLO plasmid, an origin

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Term
Fall
Professor
Molly Reilly
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