Spring 08 Exam 2 - 1362 EXAM 2 YELLOW VERSION 1 Which one...

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Page 1 1362 EXAM 2 YELLOW VERSION 1. Which one of the following pairs is not correctly associated with each other? A) Histone modification : pre-transcriptional control mechanism B) snRNP's : part of the transcription initiation complex of eukaryotes C) Ubiquitin/proteosome : post-translational mechanism D) Intron : non-coding sequence Ans: B 2. Which of the following are false concerning reproductive cloning? A) Involves the technique of nuclear transplantation B) The source of the enucleated egg is the one who is cloned. C) Reproductive cloning is not a very efficient process; success occurs only after hundreds of attempts. D) It requires a surrogate mother in which to implant the embryo. Ans: B Use the following to answer questions 3-4: A bacterial cell has the following metabolic pathway: AB C enzyme 1 enzyme 2 The genes coding for enzymes 1 and 2 in this pathway are organized into an operon. 3. Enzyme 1 gene and enzyme 2 gene are only transcribed when molecules of "A" are present; for this reason the operon would be characterized as: A) a repressible operon. B) as always being on. C) an inducible operon. D) lysogenic operon. Ans: C 4. In this operon, enzyme 1 gene and enzyme 2 gene would be referred to as the A) regulatory genes B) structural genes C) exons D) operon E) promoter Ans: B 5. A "translatable" transcript in a eukaryote must have all the following features, except : (Hint: if it is not an absolute requirement, then reject it) A) a poly A tail B) a 5' cap C) an uninterupted, continuous coding sequence D) exons in the same order as dictated by the DNA sequence that was transcribed E) A-D are all requirements a transcript must posses in order to be translated into a protein. Ans: D
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Page 2 6. In terms of gene organization and control, eukaryotic and prokaryotic cells are similar in that both possess A) promoter sequences B) regulatory proteins that can stop transcription C) multiple genes under the control of a single promoter D) Only A and B are common to both cell types. E) A, B, and C are true of both cell types. Ans: D Use the following to answer questions 7-8: Sam is an adopted child. Upon researching who his mother might be, the adoption agency provided 3 candidate mothers. RFLP analysis was carried out to perhaps identify the possible mother. Sam's DNA sample is in lane #1, samples from the possible mothers are in lanes #2, #3, and #4. The boxes at the very top represent wells into which the DNA samples were loaded. 1 2 3 4 7. Of the three possible mothers, which candidate is likely to be Sam's biological mother? A) 2 B) 3 C) 4 D) There is no exact match with Sam, thus none of these mothers is a possibility. Ans: A 8. Which of the following is false regarding this analysis? A) VNTR sequences were likely analyzed and amplified by PCR. B) A Southern blot was performed.
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Spring 08 Exam 2 - 1362 EXAM 2 YELLOW VERSION 1 Which one...

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