PHAN 212-LAB Worksheet-Ex-11 CHROMATOGRAPHY.pdf - OLFU – College of Pharmacy PHAN 2 Laboratory Exercise no. 11 Chromatography Name: Date: 6/1/22 Year

OLFU–College of PharmacyPHAN 2 LaboratoryExercise no. 11ChromatographyName:Date:6/1/22Year and Sec. PH2YB-3Group no.Materials(Basics of Chromatography)(Chromatography at Home)USP <621>:Procedure:Familiarize with the principle of Chromatography, how to operate an HPLC apparatus andidentify and discuss the function of its parts by watching the links provided. The followingprocedures reflect the experiment conducted in the video.Paper chromatography:1.Prepare all materials needed for this simple experiment (Glass, Tissue Paper, ColoredMarkers, Pencil, Ruler)2.Using pair of scissors, cut the strips of tissue paper at least 1.25 inches in width and 6inches in height.3.Draw a horizontal line 1 inch from the other side of tissue paper.4.Mark the center of horizontal line with a small dot using your colored marker. (Red, Blue,Green)Note: 3 colored markers will be needed in this experiment. This will be done separately foreach strip of tissue paper, so you will need 4 strips of tissue paper.5.Roll the other end of tissue paper at least 2 inches to the pencil and hang the pencil intothe glass.6.Add the solvent into the glass enough to soak the strips of tissue paper.Note: 3 solventswill be use: 70 % Isopropyl Alcohol, Water and Mixture of water and isopropyl alcohol7.Measure the distance travelled by the solute (colored and small dot) and the solvent.8.Compute for the Rf value using this formula.Rf value = distance travelled by solute/ distance travelled by the solventSOLUTE(COLORED DOTS)DISTANCETRAVELLEDBY SOLUTEDISTANCE TRAVELLEDBY SOLVENT 1(ALCOHOL)RF VALUE

OLFU–College of PharmacyPHAN 2 LaboratoryRED1.5 inches1.7 inches1.5/1.7 = 0.88BLUE1.0 inch1.7 inches1.0/1.7 = 0.59GREEN1.6 inches1.7 inches1.6/1.7 = 0.94SOLUTEDISTANCETRAVELLEDBY SOLUTEDISTANCE TRAVELLED BYSOLVENT 2(WATER)RF VALUE

OLFU–College of PharmacyPHAN 2 Laboratory(COLORED DOTS)RED1.2 inches2.95 inches1.2/2.95 = 0.41BLUE2.7 inches2.95 inches2.7/2.95 = 0.92GREEN2.6 inches2.95 inches2.6/2.95 = 0.88SOLUTE(COLORED DOTS)DISTANCETRAVELLEDBY SOLUTEDISTANCE TRAVELLED BYSOLVENT 3(MIXTURE OF ALCOHOLAND WATER)RF VALUE

OLFU–College of PharmacyPHAN 2 LaboratoryRED1.9 inches2.0 inches1.9/2.0 = 0.95BLUE1.5 inches2.0 inches1.5/2.0 = 0.75GREEN1.8 inches2.0 inches1.8/2.0 = 0.9*SOLVENT 170 % ISOPROPYL ALCOHOL*SOLVENT 2WATER*SOLVENT 3MIXTURE OF 70 % ISOPROPYL ALCOHOL and WATER

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PHAN 212-LAB Worksheet-Ex-11 CHROMATOGRAPHY.pdf - OLFU –...

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