lab 8_ cellular compartments

lab 8_ cellular compartments - Separation of Intracellular...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
Separation of Intracellular Compartments To measure protein concentration of each of the cellular fractions. To measure the relative activities of two enzymes which are characteristic  of two different metabolic pathways (next lab).  To isolate the cytosolic, nuclear, and mitochondrial  compartments from a crude eukaryotic cell extract. 
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
An animal cell (fig. 1.22)
Background image of page 2
Differential Centrifugation separates on the basis of size, separates a suspension in two  fractions: pellet and supernatant Centrifugation: to separate mixture of components based on size and density Pro: quick and simple Con: lack of a fine separation, possible cross contamination between fractions
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Gradient Centrifugation separates a suspension on the basis of their  buoyant densities  Pro: fine separation of many or all components in a mixture Con: time-consuming, relative difficult to carry out
Background image of page 4
1. Preparing Crude Extract. 2.
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 6
This is the end of the preview. Sign up to access the rest of the document.

This lab report was uploaded on 04/22/2008 for the course BISC 330L taught by Professor Petruska,tower during the Spring '07 term at USC.

Page1 / 10

lab 8_ cellular compartments - Separation of Intracellular...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online