Exams,Fall2000

Exams,Fall2000 - Matching: each answer (A-E) can be used...

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Page 1 BIMM120 Midterm #1 October 12, 2000 Matching: each answer (A-E) can be used more than once in a section. However, for each question, there is just one correct answer. (1-7) Match the component to the kind of cell it is found in: 1. Lipopolysaccharide (LPS) 2. In some species, a wall made of protein 3. Outer membrane 4. Thin layer of peptidoglycan 5. Thick layer of peptidoglycan 6. A nuclear membrane 7. Lipids containing ether linkages and isoprene A. Gram positive bacteria B. Gram negative bacteria C. Archaea D. Eukaryotes E. All of the above (8-10) Match the following adaptations to the environmental extreme they counteract: 8. A lipid monolayer 9. Accumulation of high concentrations of internal solutes 10. Increased proportion of saturated fatty acids A. Low temperatures B. Very high temperatures C. High salt concentrations D. High pH E. Low salt concentrations (11-14) Match the following cell features to the functions listed in A-E. 11. Pilus 12. Flagellum 13. Capsule 14. Outer membrane 15. Peptidoglycan A. When present, provides an excellent permeability barrier for hydrophobic molecules, such as penicillin and detergents B. Allows binding to surfaces or other cells C. Determines the shape of the bacterial cell D. Allow cells to swim (i.e.: confers motility) E. The site of DNA replication
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Page 2 (16-20) Match the terms below with the definitions listed in A-E. 16. Sterilization 17. Decontamination 18. Disinfection 19. Germicide 20. Pasteurization A. Reduction of pathogenic bacteria to a point where they pose no risk of infection. B. Destruction of all microbial life. C. A compound that kills microorganisms. D. Physically removing microbes from an instrument or surface. E. A compound that prevents microbial growth, but does not kill microbes. (21-25) You are performing a growth curve on your favorite bacterium, so you start a liquid culture and periodically measure cell number. You plot time versus cell number (which is plotted on "log" paper), and obtain the following graph: time A B C D 10 7 10 8 10 9 10 6 Match the phase of growth indicated by the letters on the graph to the corresponding description: 21. Exponential growth. 22. The synthesis of proteins required for survival in stationary phase starts here. 23. This phase reflects the time required for stationary phase (or "old") cells to resume growth. 24. The rate of DNA and protein synthesis is highest during this phase of growth. 25. Toxic products of metabolism may have accumulated and completely inhibited growth.
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Page 3 (26-28) Imagine that you are a bacterium that has recently been ingested by a human, and that your goal is to colonize (i.e.: grow in) the large intestine. Select a tool from A-E that will help you surmount the obstacles listed in 26-28, and allow you to reach your goal: Obstacles: 26. The detergent-like bile salts found in the stomach, which could destroy your cytoplasmic membrane. 27.
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Exams,Fall2000 - Matching: each answer (A-E) can be used...

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