Separation of Plant Pigments Abstract-2

Separation of Plant Pigments Abstract-2 - Sarah Abdul...

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Sarah Abdul, Spring 2008 Plant Physiology Class, Arizona State University, Identification of plant pigments in spinach leaves via column chromatography. The majority of plants contain chlorophyll a and b, carotenes, and xanthophylls, all of which contribute to the color of plant leaves and algae. The chemical structure, which is different for each pigment, allows the pigments to absorb light at different frequencies by altering their solubility characteristics. The lab group was able to separate each pigment from one another, and analyze each separation sample, to determine the type of pigment present. The group separated the pigments by using chromatography, on a column of fine powdered sugar. To determine the type of pigment present, the group isolated each separation phase in test tubes and then analyzed it using absorption spectra. It was observed that xanthophylls pigments had the least amount of solubility, and absorb mainly in the blue region, with peaks at λ= 642.5. Chlorophyll b pigments
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This note was uploaded on 04/23/2008 for the course PHY 111 taught by Professor Cavatto during the Spring '08 term at ASU.

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Separation of Plant Pigments Abstract-2 - Sarah Abdul...

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