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BIO 200 Homework 8 Chapter 8 summer 2022.docx - BIO 200:...

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BIO 200: Homework 82 pointsFor the homework assignment, you must answer the questions marked by bold andhighlighting(0.5 pt each). You may answer more which will be read but will not affect the grade as thisassignment doubles as a study guide.Chapter 8: Recombinant DNA TechnologyThe Role of Recombinant DNA Technology in Biotechnology1.Describe the three main goals of scientists who practice recombinant engineering.The Tools of Recombinant DNA Technology2.Describe what mutagens are and how scientists use them in DNA technology.3.Describe the function of reverse transcriptase. Be sure your answer includes the words RNA and DNA.4.What is cDNA? Describe how cDNA is different from the DNA found in a cell’s nucleus. Also, describe howscientists can use cDNA and why.5.Describe what DNA probes are and how they work. Also, describe what scientists can learn from using aparticular probe. What is a specific target that might be identified using a DNA probe?6.Describe what restriction enzymes are and their function. Be sure your answer includes explanations of thefollowing terms: restriction sites, sticky ends, blunt ends, and ligase.7.Describe what a vector is with regard to genetic engineering (what scientists use it for).8.Describe what gene libraries are and what they are used for.Techniques of Recombinant DNA Technology[For all techniques, be able to recognize an example of the technique in use. Recognize what makes each techniquedifferent from the other techniques]9.Explain what PCR is and generally describe the steps of PCR (answer should include definition/explanation for
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molecular biology, DNA, Recombinant DNA technology

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