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Antibiotics and Antiseptics

Antibiotics and Antiseptics - Travis McMaine BIO 153-011...

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Travis McMaine BIO 153-011 Experiments examining the effects of antibiotics, antiseptics and disinfectants on bacterial growth Introduction In this lab three types of inhibitors are used: antibiotics, antiseptics, and disinfectants. Antibiotics are chemotherapeutic agents that prevent or destroy microbes (specifically bacteria, fungi, and protozoan). Antiseptics are applied to living tissue to kill microbes. Disinfectants are used to destroy microorganisms on non-living surfaces. The antibiotics used in this lab were Oxacillin (OX), Sulfamexathoxazole with Trimethroprim (SXT), Penicillin (P-2), Gentamicin (GM-10), Cephalothin (CF-30), Erthromycin (E-15), Ciprofloxacin (CIP-5), and Novabiocin (NB-30). The antiseptics used were Rubbing Alcohol, Hydrogen Peroxide, Listerine, Baking Soda, and Bactericidal Soap. The disinfectants used were Clorox, Lysol, Ammonia, and Vinegar. One negative control was used and it was Distilled Water. The bacteria tested in the lab were Escherichia coli (Sample 1), Pseudomonas aeruginosa (Sample 2), and Staphylococcus aurelius (Sample 3). I predict that all three bacteria will be susceptible to Ciprofloxacin. This is because it is a commonly used, strong, and broad-spectrum antibiotic. I also predict that Gram negative and positive bacteria will be affected by the antiseptics Alcohol, Hydrogen Peroxide, and Bactericidal Soap. This are all commonly used to clean minor cuts so they should be effective against both types of bacteria. Lastly, I predict that the disinfectants Lysol and Bleach are very effective against both types of bacteria. This is because both of these commercial products claim in there advertisements that the kill 99.9% of bacteria. Materials and Methods See lab Manuel pages 137-161 Results Table 1 Organism Name Shape Gram +/- 1 E. coli Bacillus Negative 2 P. aeruginosa Bacillus Negative 3 S. aurelius Coccus Positive Table 1 Antibiotics
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Organism OX SXT P-2 GM-10 CF-10 E-15 CIP-5 NB-30 1 (gram -) 0.5 30.83 1.66 19.17 20.5 12 36.67 0 2 (gram -) 0 0 0 26.6 0 0 31.6 0 3 (gram +) 25.4 29.8 32.6 23 40.2 25.6 26.2 24 Table 2 Antiseptic Organism Alcohol Peroxide Listerine Baking  Soda
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