handout 3

handout 3 - Alan Chuang (mchuang@ucsd.edu) BIMM120 WI08 OH...

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Alan Chuang ( mchuang@ucsd.edu ) BIMM120 WI08 OH : Wed 3 :30-4 :30 Cafe Roma 2/1/2008 Handout 3 Cell growth o Why are there different phases of growth in a microbial cell? o In what instances, will you find the three different stages? o Is growth rate linear with respect to concentration of an essential substrate? [low] vs. [high] o How do you maintain a culture within exponential growth? Chemostat can be used to maintain exponential growth How is a chemostat a self-correcting system? o Ribosome content within cells Why is it not linear? advantage? o Temperature effect on growth What kind of microbe will have optimum growth at a low temperature? Cell division o DNA replication Multi-fork replication Does bacteria only divide if all replication is completed? Can they have partially replicated DNA after segregation? How about eukaryotes? Gene dosage Will multi-fork replication cause gene dosage to vary? What is important then about positioning of a gene between the origin and terminus? Rate of DNA replication Frequency of initiation or rate of DNA replication? o Gram – vs. gram + o Septum formation FtsZ forms constricting ring called Z-ring where the cell septum will form Z-ring closes like iris of the eye
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handout 3 - Alan Chuang (mchuang@ucsd.edu) BIMM120 WI08 OH...

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