worksheet 8

worksheet 8 - Worksheet 8 Enzymes = increases rate of...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Worksheet 8 1 Enzymes = increases rate of chemical reactions by: 1. 2. 3. k d k m = ½ V max = affinity of enzyme for substrate k cat = rate of final conversion into product k cat /k m = rate of overall reaction _______________________________________________________________________ _ Proteases (aka Proteinases) – generally responsible for peptide cleavage. 4 Families: Ser, Cys, Asp, Metallo Named after the most functional reactive species in the active site. Serine Proteases Tetrahedral Intermediate Ex. Chymotrypsin, Trypsin 4 Characteristics: 1. Catalytic Triad: Asp, His, Ser a. Ser: most reactive residue, in contact with substrate i. OH group responsible for attack on substrate. b. His + : stabilizes transition state i. Donates and accepts free H + c. Asp - : compensates charge on His + . 2. Oxyanion Hole: contains an Asn (polarizable) that also interacts with O - of intermediate state (like ____ does) to stabilize the transition state overall. 3. Nonspecific Binding: responsible for _____________________________ ___________________________________________________________. 4. Specificity Pocket: unique to each enzyme. Responsible for specificity between enzyme families. Peptide Cleavage by Serine Proteases is a two step process:
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 4

worksheet 8 - Worksheet 8 Enzymes = increases rate of...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online