Respiration Photosinthesis In Plants

Respiration Photosinthesis In Plants - Respiration and...

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Respiration and Photosynthesis in Plants Lab exercise adapted from http://northonline.sccd.ctc.edu/bio200s/bio201/index.html Objectives : In this lab exercise, you will: Use a pH probe to measure the pH of water. Use pH measurements to make inferences on the amount of carbon dioxide in water. Use the inferences about the amount of carbon dioxide in water to make conclusions about whether plants consume, or produce carbon dioxide in light. Use the inferences about the amount of carbon dioxide in water to make conclusions about whether the plant is respiring or photosynthesizing more in light. Reinforce concepts about respiration and photosynthesis. Introduction We can measure the rate of respiration in several ways, all of which come from the basic equation of respiration: C 6 H 12 0 6 + 60 2 6 H 2 0 + 6 CO 2 + energy Thus, we can measure the following: 1. rate of disappearance (consumption) of glucose 2. rate of the disappearance (consumption) of oxygen 3. rate of production of water 4. rate of production of carbon dioxide 5. rate of the production of energy Because we are measuring respiration in living organisms, it is not easy to measure the consumption of glucose, or the production of water or energy. Energy produced is captured as ATP, and some of it is lost as heat. The easiest things to measure are the consumption of oxygen and the production of carbon dioxide. In this lab, we will concentrate on the production of carbon dioxide. To perform the tests, you need to determine the presence of carbon dioxide. Respiring aquatic organisms release carbon dioxide directly into their watery environments. When carbon dioxide dissolves in water, it forms carbonic acid, H 2 CO 3 , and the pH decreases. If carbon dioxide is removed from pond water, the amount of carbonic acid goes down, and the pH increases. Therefore, we have an indirect measure of the amount of carbon dioxide in the water: the pH of the water. A pH probe can be used to monitor pH and determine whether carbon dioxide is released into the water, or is removed from the water. Since plants are composed of cells, and all cells respire, we can measure respiration in
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Respiration Photosinthesis In Plants - Respiration and...

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