BMI_Lab7BacterialTransform

BMI_Lab7BacterialTransform - Session 7 is the Mid-Term...

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Session 7 is the Mid-Term (1hour) Laboratory VII (2 hours) Topic: Bacterial Transformation, Isolation of DNA Introduction : A plasmid is an extra chromosomal piece of circular DNA, which is replicated in bacteria. Plasmids are also transferable from bacteria to bacteria. pUC 19 plasmid used in this transformation carries an ampicillin resistant ( β -lactamase) gene. β - lactamases can hydrolyze ampicillin and render it ineffective . Bacterial cultures are treated so that the cells become competent to take up DNA from the media environment by the process of transformation . Competent cells have weakened cell walls and pores that allow the transfer of plasmids into the bacteria. The cells used for this experiment are DH5 α cells. DH5 α is a genetically modified Escherichia coli strain. C ompetent DH5 α cells are can be directly purchased from the manufacturer. Transformed cells can be selected after plating in the respective antibiotic media. Only cells transformed with the antibiotic resistant plasmid will grow in the antibiotic media. All other cells will be killed
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This note was uploaded on 04/24/2008 for the course PHS 2301 taught by Professor Carvalho during the Spring '08 term at St. Johns Duplicate.

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BMI_Lab7BacterialTransform - Session 7 is the Mid-Term...

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