BMI_Lab6EnzymePhysical - Laboratory IV Topic: Enzyme...

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Laboratory IV Topic : Enzyme Physical Properties Introduction : Enzymes are important for biological function and are their activity varies with pH and temperature. Most enzymes in the human body are active at 37 ° C at a neutral pH. Some enzymes are active at extreme conditions. Acid phosphatase works in acidic conditions while alkaline phosphates require an alkaline pH for its activity. Enzymes of thermophilic bacteria have enzymes that are active at high temperatures (greater than 45 ° C). Psychrophilic organisms need enzymes that work efficiently at very low temperatures. Thermophilic and psychrophilic enzymes are useful in biotechnology (eg. DNA polymerases) Enzyme also exhibit substrate specificity. Last week we studied acid phosphatase enzyme that hydrolyzed phosphate bonds. In today’s exercise we will study the effect of pH and temperature on amylase activity and the specificity of amylase. Amylases are present in human saliva and in bacteria, fungi, and plants. Amylases beaks down starch polymer to smaller maltose units by hydrolyzing the alpha-1,4 and alpha-1,6 glucosidic bonds. This hydrolyses can be visually detected by the addition of iodine. Iodine (yellowish brown) reacts with intact starch to form a blue color complex. Hydrolyzed starch does NOT show blue color with iodine. Starch +
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BMI_Lab6EnzymePhysical - Laboratory IV Topic: Enzyme...

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