Domain Bacteria Lab Report 120

Domain Bacteria Lab Report 120 - A Study of the Variety of...

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Introduction A Study of the Variety of Prokaryotes within the Domain Bacteria Soyun Kim
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In this laboratory experiment, we studied the variety of prokaryotes included in the Domain Bacteria. In comparison to the Domain Eukarya, Bacteria are much smaller in size, lack membrane-bound organelles, and their DNA is kept within a nucleoid rather than a true nucleus. Some of the major groups of bacteria include the cyanobacteria and the eubacteria, and these are the bacteria that we studied in our experiments. Cyanobacteria can exist in extreme environments lacking organic nutrients, carrying out photosynthesis. The Eubacteria are characterized as coccus (spherical), bacillus (rod), spirillum (spiral), or vibrio (common shape). Materials and Methods Experiment 1 In our first experiment, we examined a drop of Anabaena culture on a slide under high power in our compound microscope at 40 times magnification. Experiment 2 In the second experiment we prepared a prepared slide of bacteria under oil immersion and examined it under our compound microscope at 100 times magnification. Experiment 3 In the third experiment we cultured bacteria in an agar medium in a Petri plate. 4 agar plates and a marker were used. We labeled one plate “air” and placed the plate near a source of moving air and left it fully open for 5 minutes. We then closed the plate and placed it in an incubator at 17 degrees Celsius for 48 hours. We labeled the second plate “cough” and one person coughed on the plate three times with their mouth open. We then closed the plate and placed it in an incubator at 17 degrees Celsius for 48 hours. We labeled the third plate “suppressed cough”. One
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person coughed over the plate three times while keeping their mouth closed. We then closed the plate and placed it in an incubator at 17 degrees Celsius for 48
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This note was uploaded on 02/27/2008 for the course BISC 120Lg taught by Professor 11:00-01:50pm during the Fall '06 term at USC.

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Domain Bacteria Lab Report 120 - A Study of the Variety of...

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