Biology Lab Report 2

Biology Lab Report 2 - The Concentration of Salmonella...

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The Concentration of Salmonella Typhirium Antibody in blood serum measured by an Enzyme-Linked Immunosorbent Assay
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Gans 2 The Concentration of Salmonella Typhirium Antibody in blood serum measured by an Enzyme- Linked Immunosorbent Assay Introduction By using known a specific antibody to determine the presence of its respective antigen, diseases are able to be diagnosed. Immunoassays such as the ELISA technique (enzyme-linked immunosorbent assay), are based on our knowledge of how antibodies react with their corresponding antigens. ELISAs use enzymes because the results of the experiment are able to be calculated quantitatively and qualitatively. An enzyme’s nature is such that, with greater concentration and excess of substrate, a greater amount of product is formed; in this way the results of the ELISA are measured quantitatively for the amount of antibodies in blood serum. The enzymes horseradish peroxidase and alkaline phosphatase are most often used and, upon substrate addition, a colored product is formed and is able to be detected without visual aid or, for precise quantitative results, with the aid of a spectrophotometer. In the Indirect ELISA, a known antigen is used to detect the presence of a specific antibody; the opposite is true for the direct ELISA where a known antibody is used to detect the presence of an antigen. In this experiment the Indirect ELISA technique was used. Furthermore in the Indirect ELISA, the enzymes are linked to the anti-antibodies. It is upon connection by the anti-antibody to the antibody in the serum that the linked enzyme is activated and able to start catalyzing the substrate to form the colored product that will be the quantitative indicator
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Biology Lab Report 2 - The Concentration of Salmonella...

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