ENGR213_Lecture7

ENGR213_Lecture7 - Electrophoresis BRAE 213 Lecture 7...

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Unformatted text preview: Electrophoresis BRAE 213 Lecture 7 Electrophoresis Background Biological Species Have Charge: • protein, • cells, • DNA, • Virus, etc… Surface Charge can be dependent on background solution ionic strength and pH Electrokinetic Theory (Electro-neutrality)- Charged Surface------------------- + + + + + + + + + + + + + + + + + + + +--- + S u r f a c e P o t e n t i a l δ ζ ψ The Electric Double Layer • ψ , dry surface potential • ζ , the zeta potential or realized potential • δ , thickness of the Stern layer • κ D , approximate thickness of the diffuse layer κ D- 1 κ D- 1 ≤ 1 microns Electrokinetic Theory-------------------- + + + + + + + + + + + + + + + + + +--- + ζ = + + + + + +------ κ D- 1 Electro-Osmotic Flow---- + + + + E Slip Velocity at the Surface U S =- εζ E x μ EO Flow • Electric field acts on ions in the Electric double-layer • Flow driven by fluid entrainment, ion transport • Fluid appears to “slip” at surfaces at U S Electrophoresis...
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ENGR213_Lecture7 - Electrophoresis BRAE 213 Lecture 7...

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