ENGR213_Lecture8_BRAE213

ENGR213_Lecture8_BRAE213 - Qiuzzes: Contact Mike Ponder...

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Lecture 8 Immunoassay Biotechnology Qiuzzes: Contact Mike Ponder mponder@calpoly.edu
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Business Finals Schedule • BIO 213-01 (T 4-6) is "partnered" with  ENGR/BRAE 213-72 (R 4-6) and their scheduled  final should be on Thursday, March 20, 7-10  p.m. in 6-124  • BIO 213-02 (W 4-6) is "partnered" with  ENGR/BRAE 213-71 (M 4-6) and their  scheduled final should be on Wednesday,  March 19, 4-7 p.m. in 6-124 
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New Definitions Antibody  -- A class of serum proteins that are induced by  exposing to an immunogen (antigen) and that binds  specifically to the antigen/analye forming an  antibody/antigen complex Antigen  -- A molecule that can be selectively bound by an  antibody. Not always immunogenic. Immunogenic  -- evoking an immune response Antiserum  -- Serum from an animal containing specific  antibodies Chromophore  -- A chemical that changes color, resulting  from Enzyme interaction with a substrate. Conjugate  -- Formed by covalently coupling two  molecules, e.g., antibody with an enzyme
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Some New Definitions Cross-Reactivity  -- The ability of antibodies to react with  other compounds other than the target antigen  ELISA  -- Enzyme-Linked immunosorbent assay (ELISA)  commonly used for environmental monitoring Enzyme  -- A substance that can react at a low  concentration as a catalyst promoting reaction Enzyme-Conjugate -- An enzyme that is covalently  coupled to a protein such as an antibody or to a target  analyte/hapten Hapten  -- A small antigenic molecule that can bind to an  antibody, does not induce antibody immune response.
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Some New Definitions IgG  -- A divalent immunoglobulin-G antibody molecule Immunogen  -- A substance that can generate a strong  immune response (antigen) Monoclonal Antibody  -- Homogeneous antibody  population produced by cloning a single B-cell line with  identical selectivity and affinity Polyclonal Antibody  -- An antibody population with  heterogeneous selectivity and affinity produced by many  clones of B-cells  Substrate  -- A chemical that reacts with an enzyme Titer -- A dilution of a substance that produces a desired  concentration, e.g., concentration of antibody in an  immunoassay
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ENGR213_Lecture8_BRAE213 - Qiuzzes: Contact Mike Ponder...

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