BMI_Lab2HPLC_pH - High Performance Liquid Chromatography pH...

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High Performance Liquid Chromatography Lab 2
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Activity 1 pH Detection
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pH pH is negative log of hydrogen ion concentration pH changes due to increase dissociation of acids or bases in solution Some ways to measure pH pH meter - instrumental pH indicator -colorimetric
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pH indicators Some weak acids exhibit different colors in a ionized and ionized form Nitrophenol ionizes at pH >10 unionized form is colorless Ionized form is yellow These compounds are useful for colorimetric pH detection A universal pH indicator contains a mixture of pH indicators to show changes in color over a wide range of pH
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pH of Carbonate Buffers The pH of CO 3 buffer changes with the concentration (moles) of Carbonate and Bicarbonate present in solution. Today’s Experiment Prepare stock solutions for buffers NaCO 3 and NaHCO 3 (0.2M) Prepare pH buffers by mixing different volumes of the stock solutions Measure pH of the buffers using pH meter Compare color changes of the buffers with universal pH indicator at the different pH
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High Performance Liquid Chromatography Activity 2
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Objectives To understand the principles High Performance Liquid Chromatography (HPLC) To find the retention times of three drugs, Salicylic acid, Phenobarbital and Phenytoin using HPLC To identify the compound(s) present in a unknown sample.
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This note was uploaded on 04/24/2008 for the course PHS 2301 taught by Professor Carvalho during the Spring '08 term at St. Johns Duplicate.

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BMI_Lab2HPLC_pH - High Performance Liquid Chromatography pH...

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