Practice_Exam3_fromF07

Practice_Exam3_fromF07 - Molecular Genetics 382 Practice...

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Molecular Genetics 382 Practice Exam 3 1) Briefly compare and contrast the Group I and Group II self-splicing mechanisms (describe one major similarity and one major difference). i) Similarity (2 pts): ii) Difference (2 pts): 2) Each of the items in list A recognizes one of the items in list B. Match each item in list A with the appropriate item in list B. (6 pts) List A: match from list B (just give letter) : 1) U1 snRNP 1) ________ 2) U2 snRNP 2) ________ 3) U4/U6-U5 snRNP 3) ________ List B: a) 3’ splice site b) 5’ splice site c) branch site Page 1 of 4
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3) The following are the results of an in vivo splicing assay with different mutants of a certain yeast gene. The black and white boxes are exons, the lines are intronic sequences, and the cross-hatched box is a conserved sequence found in all yeast introns. What are the two major conclusions that can be drawn from this experiment and its results? (6 pts) Conclusion 1: Conclusion 2: Page 2 of 4 Spliced AG AG Wild Type Mutant # 1 Mutant # 2
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Practice_Exam3_fromF07 - Molecular Genetics 382 Practice...

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