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biochem1exam2008withoutanswers - Name(Print Last First...

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Name ( Print Last, First )__________________________________________________ FIRST EXAMINATION Biology 366 and Chemistry 361/461 February 7, 2008 INSTRUCTIONS : 1) You are responsible for printing your name legibly at the top of this page. 2) You must use a number two pencil to enter your answers in the scantron . 3) Make sure that the I.D. number, name (last and first) and test form (use form A) fields are properly filled. The I.D. number field should contain ten digits - you may choose a variation of your Student’s ID number, SSN or phone number starting with the area code. 4) At the end of the test , insert the scantron inside the examination, and return both to the proctor. Question Type Actual Score Maximum Multiple Choice 100 Adjusted Score (if applicable) 100 Mean ± SD Exam Grade 1
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MULTIPLE CHOICE QUESTIONS (40 questions total; 2.5 points each) Questions 1 through 28 are based on the material covered in the lectures by Dr. Freitas, and the remaining 12 questions focus on topics taught by Dr. Castignetti. SELECT THE MOST CORRECT ANSWER FOR EACH MULTIPLE-CHOICE QUESTION 1. The polarity of water is due to 1. the tetrahedral bond angle of H-O-H. 2. the less than tetrahedral bond angle of H-O-H. 3. the high electronegativity of oxygen. 4. the high electronegativity of hydrogen. A) only 1 B) only 2 C) 1 and 3 D) 2 and 3 E) 2 and 4 2. The pK a of a weak acid is 4.7, and the pH of a 1 L buffer solution prepared from this acid is also 4.7. Which of the following statements are true after a drop of 0.1 M NaOH is added to the solution? 1. The solution will become slightly more acidic. 2. The solution will become slightly more basic. 3. The [base]/[acid] ratio will increase slightly. 4. The [base]/[acid] ratio will decrease slightly. 5. The [base]/[acid] ratio will remain the same. A) only 1 B) 1 and 4 C) 2 and 3 D) 2 and 4 E) 2 and 5 3. HEPES, which stands for N-(2-hydroxyethyl)-piperazine-N'-2-ethanesulfonic acid, has a pK a value of 7.55 and is commonly used in biochemical laboratories to prepare buffers. To 5.0 mL of 0.01 M HEPES buffer, pH 7.55, a biochemistry graduate student adds 5.0 μL of 1.0 M HCl. The final pH was A) 3.55 B) 6.46 C) 7.37 D) 7.55 E) 7.64 4. Hydrogen bonds can be formed between all of the following except A) N-H and HO-R B) O-H and HO-R C) C-H and HO-R D) C=O and HNRR’ E) O-H and H 2 O 5. The most prevalent type of noncovalent interaction in proteins is a(n) _______ bond. A) hydrogen B) hydrophobic C) peptide D) ionic E) disulfide 6. At physiological pH, an amino acid with two carboxyl groups of pK a = 2.0 and pK a = 4.0 and one amino group of pK a = 10.0 will have a charge of ___(a)___ .The isoelectric point of this amino acid is closest to ___(b)___ . A) (a) -1; (b) 3.0
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