2008Quiz2withanswers

2008Quiz2withanswers - At a concentration of 2.0 mg/mL in...

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Chem 361/461 and Bio 366 Quiz 2 INSTRUCTIONS : A) You must use a number two pencil to enter the answers in the scantron . B) Make sure that the I.D. number, name (last and first) and test form (use form A) fields are properly filled. The I.D. number field should contain ten digits - you may choose a variation of your Student’s ID number, SSN or phone number starting with the area code. 1. What is the common strategy by which catalysis occurs? A) increasing the probability of product formation. B) shifting the reaction equilibrium. *C) stabilization of the transition state. D) all of the above. E) none of the above. 2. When substrate concentration is greater than K M , the rate of catalysis is equal to A) K d B) k cat *C) V max D) all of the above E) none of the above For questions 3 through 5 below, consider the following data for the Lineweaver-Burk plots obtained for a homotetrameric enzyme with a molecular weight of 40,000 D.
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Unformatted text preview: At a concentration of 2.0 mg/mL in the absence and presence of 0.10 mM of an inhibitor, the plots crossed at the x-axis at – 5.0 mM-1 and showed two y-intercepts at 1.0 x 10-4 M-1 .min and 2.0 x 10-4 M-1 .min. 3. What type of inhibitor is present? A) A competitive inhibitor. *B) A non-competitive inhibitor. C) An uncompetitive inhibitor. D) A suicide inhibitor. E) A catalytic antibody. 1 4. The values of K M and V max are, respectively, A) 5.0 mM-1 ; 1.0 x 10-4 M-1 .min B) 1.0 mM-1 ; 1.0 x 10-4 M-1 .min C) 0.50 mM; 1.0 x 10-4 M-1 .min D) 0.20 mM; 1.0 x 10 4 mM.min-1 *E) 0.20 mM; 1.0 x 10 4 M.min-1 5. The values of K I and k cat are, respectively, *A) 0.10 mM; 5.0 x 10 7 min-1 B) 0.10 mM-1 ; 1.0 x 10 4 M.min-1 C) -0.20 mM-1 ; 5.0 x 10 7 min-1 D) 0.20 mM; 1.25 x 10 4 min-1 E) 0.50 mM-1 ; 5.0 x 10 3 M.mL.mg-1 . min-1 2...
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This note was uploaded on 04/26/2008 for the course CHEM 361 taught by Professor Defreitas during the Spring '08 term at Loyola Chicago.

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2008Quiz2withanswers - At a concentration of 2.0 mg/mL in...

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