07exam2key - Gene Action Exam 2 KEY Winter 2007 Name:_ 1....

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Gene Action Exam 2 KEY Winter 2007 Name:_____________________ ox rep rep 1. (20 pts) Diagram the most common series of events leading to increased mutation for the mutator strains listed below. The events leading to increased G:C T:A mutations in mutM mutants are shown. Show only the DNA which becomes mutated, use the fewest steps possible, and label the steps (“ox” = oxidation; “rep” = replication; “rpr” = repair; “MutY” = MutY action; “MutM” = MutM action; “MutT” = MutT action). (Note: “O” = 8-oxoguanine.) (Remember that no repair system is 100% efficient.) mutM —AC— —AC— —AA— —AA— —TG— —TO— —TO— —TT— a. mutT rep MutY MutM —AC— —AC— —CC— —CC— —TG— —OG— —OG— —GG— b. mutY ox rep rep —AC— —AC— —AA— —AA— —TG— —TO— —TO— —TT— c. mutT mutY rep rep MutM —AC— —AC— —CC— —CC— —TG— —OG— —OG— —GG— d. mutM mutY ox rep rep —AC— —AC— —AA— —AA— —TG— —TO— —TO— —TT— e. mutS (G:C A:T change) —AC— —AC— —AT— —TG— rep —TA— rep —TA— or —AT— —TG—
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2. (20 pts) Imagine a donor DNA molecule carrying the replicative transposon Tn3 and a linear recipient molecule carrying a Tn3 res site (but no other Tn3 sequences) as shown at the right.
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This note was uploaded on 04/26/2008 for the course MICROM 411 taught by Professor Gray during the Winter '08 term at University of Washington.

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07exam2key - Gene Action Exam 2 KEY Winter 2007 Name:_ 1....

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