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07exam2 - Gene Action Exam 2 Winter 2007 Name 1(20 pts...

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Gene Action Exam 2 Winter 2007 Name:_____________________ ox rep rep 1. (20 pts) Diagram the most common series of events leading to increased mutation for the mutator strains listed below. The events leading to increased G:C Æ T:A mutations in mutM mutants are shown. Show only the DNA which becomes mutated, use the fewest steps possible, and label the steps (“ox” = oxidation; “rep” = replication; “rpr” = repair; “MutY” = MutY action; “MutM” = MutM action; “MutT” = MutT action). (Note: “O” = 8-oxoguanine.) (Remember that no repair system is 100% efficient.) mutM —AC— —AC— —AA— —AA— —TG— —TO— —TO— —TT— a. mutT —AC— —TG— b. mutY —AC— —TG— c. mutT mutY —AC— —TG— d. mutM mutY —AC— —TG— e. mutS (G:C Æ A:T change) —AC— —TG—
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2. (20 pts) Imagine a donor DNA molecule carrying the replicative transposon Tn3 and a linear recipient molecule carrying a Tn3 res site (but no other Tn3 sequences) as shown at the right. a. A single transposase (TnpA)-mediated event joins the two DNAs at the site indicated by the vertical arrow. The joining can occur in either of two orientations. Draw the two possible joined molecules, showing res site orientations and DNA labels (A-C, 1-4).
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