unknown #2

unknown #2 - Introduction: This lab was intended to...

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Introduction: This lab was intended to differentiate between Salmonella and Shigella . The unknown 2 is a broth mixture of Proteus , E. coli , and either Salmonella or Shigella . There are ways to test for factors that will help determine which organism is present. Those three ways are to eliminate organisms that ferment lactose, to eliminate organisms that do not ferment glucose only, and to eliminate organisms that ferment urea and to determine motility. Procedure: In the first test the objective will be to eliminate organisms that ferment lactose. This is done because Salmonella and Shigella do not ferment lactose and E. coli does ferment lactose. Three different media were used to tell which colonies have Salmonella / Shigella as opposed to E. coli . Lactose fermenters absorb the dye in the agar plates. The first media was MacConkey, which Salmonella / Shigella and other non-lactose fermenters appear smooth and colorless. Lactose fermenters appear red or dark-centered colonies. The second media was Hektoen Enteric and
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unknown #2 - Introduction: This lab was intended to...

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