{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

unknown #4 - Coagulase Test Medium This test is to see if...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Rory McPherson Unknown #4 4/14/08 Kendra Harris Introduction: This was an unknown experiment intended to differentiate between 3 types of Staphylococcus. The 3 types were: S. aureus, S. epidermis, and S. saprophyticus. In order to separate out which organism was present, 4 tests were performed. The tests were: Alpha Toxin, Mannitol, Coagulase, and Novo Biocin. Procedure: Alpha Toxin test: This test is intended to show if the organism produced an α-toxin that causes a wide, clear zone of beta hemolysis on the blood agar plate. Only S. aureus produces α -toxin, so this test is vital to determining, with certainty, which organism you have. Mannitol Salt Agar: This test is for selective isolation of coagulase-positive, mannitol- fermenting Staphylococcus . Mannitol fermentation by pathogenic staphylococci is indicated by a yellow halo surrounding the colonies. Sodium chloride is the inhibitory agent. Phenol red is the pH indicator.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Background image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Coagulase Test Medium: This test is to see if the organism causes coagulation. It contains citrated rabbit plasma which clots in the presence of the enzyme coagulase. Results: S. aureus S. epidermis S. saprophyticus Unknown #4 Alpha-Toxin +------Mannitol +--+--Coagulase +------Biotin Growth--+ NS + Novo Biocin + S R S After reviewing the results a determination was made that Unknown #4 was in fact S. epidermis. Conclusion: The intention of this experiment was to differentiate between 3 strains of staphylococci. In order to do this 4 tests were performed and the results were as shown. After reviewing all the results and comparing to known data, the unknown organism in tube #13 was found to be S. epidermis. The tests performed provided results that were felt to be accurate and this experiment should be considered a success with minimal error present....
View Full Document

{[ snackBarMessage ]}