Unknown test #3

Unknown test #3 - Introduction This is an experiment...

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Introduction: This is an experiment intended to classify an unknown organism through a series of tests that will separate out particular physiological characteristics of that organism in order to determine what it is. There were 5 control organisms used in this test, they were, Bacillui subtilis, Escherichia coli, Staphylococcus aureus, and Proteus vulgaris . For this experiment unknown tube #5 will be used. Procedure: There were 8 tests used, each test was directed at determining a physiological characteristic of the unknown organism. The tests are as follows: 1. Starch Hydrolysis: starch is a complex molecule consisting of Amylose, a straight chain polymer consisting of 200-300 glucose units. Starch also contains Amylopectin, which is a larger branched polymer containing phosphate groups. In order for bacteria to hydrolyze starch, an enzyme named Amylases will need to be produced. The unknown sample is streaked on a starch plate and after growth occurs, iodine is added to the plate. Iodine turns starch a very dark color, however, if the organism hydrolyzes the starch, there will be a clear zone around the colony, a clear zone indicates a positive test
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Unknown test #3 - Introduction This is an experiment...

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