concentrationlab3 - File: Date: Exp: Group: = enz 1 JM...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: File: Date: Exp: Group: = enz 1 JM 9.1.06.xls 1/11/2007 Activity of acid phosphatase over time 1.84E+04 L enz/tube = time = variable concentration 10 10 10 5 5 5 2.5 2.5 2.5 1 1 1 0.5 0.5 0.5 0.25 0.25 0.25 50 10 A 400 0.838 0.847 0.098 0.751 0.803 0.048 0.536 0.545 0.026 0.418 0.495 0.012 0.343 0.369 0.008 0.233 0.276 0.007 mean 0.843 corrected Units/mg 0.745 1.82E+01 tube 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 0.777 0.541 0.729 0.515 1.78E+01 1.26E+01 0.457 0.356 0.445 0.348 1.09E+01 8.51E+00 0.255 0.248 6.05E+00 Vmax = 18 Km=.75 20.00 18.00 16.00 14.00 12.00 Units/mg 10.00 8.00 6.00 4.00 2.00 0.00 0 1 2 3 4 5 6 Units 10.00 8.00 6.00 4.00 2.00 0.00 0 1 2 3 4 5 Concentration 6 0.18 0.16 0.14 0.12 1/V (mg/Unit) 0.10 0.08 0.06 0.04 0.02 0.00 0 f(x) = 0.03x + 0.06 R = 0.97 0.5 1 1.5 2 2.5 3 3.5 4 1/[S] (1/mM) concentration (mM) 10 5 2.5 1 0.5 0.25 1/[S] 0.1 0.2 0.4 1 2 4 Units/mg 18.21 17.83 12.58 10.87 8.51 6.05 1/V 0.05 0.06 0.08 0.09 0.12 0.17 Units/mg 4 5 6 7 8 9 10 Units/mg 4 5 6 7 8 9 10 Concentration Vmax= 16.9779 Km= .466893 1/V Linear Regression for 1/V 3 3.5 4 4.5 ...
View Full Document

This note was uploaded on 04/21/2008 for the course BIO 214 taught by Professor Stockton during the Spring '06 term at James Madison University.

Ask a homework question - tutors are online