simII notes 4.20.08

simII notes 4.20.08 - or else the cell would be dead CPS...

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Simulation II due May 4 th !!!!!!!!! CPS ?: Which of following are dilysogens? A. MuD1 B. MC4100 C. Mal103 MuD1: araD - , lac - , amp s , name of a phage MC4100: name of other E.Coli strain Mal103: dilysogen because lysogen means cell that contains phage; Mal103 contains MuD1 and mUC ts MC4100 gets infected by mUC ts ? You make MuD1 when you induce Mal103, also make mUC ts , also infect MC4100 with mUC ts . They don’t have selectable marker (bla; confers amp R ): If cell gets infected by mUC ts , it dies. CPS ?: Red colonies on Ara Mac + Amp plates are: A. Arabinose resistant lysogens B. Ara + mutants C. Not MuD1lysogens Not MuD1 lysogens: because they’re growing in ampicillin, they are not this choice Arabinose resistant lysogens: they are using sugar, so they’re red; create acid and metabolize sugar; if they don’t metabolize sugar, they’d be white. So they’re not Arabinose resistant lysogens because if MuD1 inserted in any genes, it couldn’t be ara + . Ara+ mutants: It isn’t in Arabinose operon, but the cell must be inserted somewhere else
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Unformatted text preview: or else the cell would be dead. CPS ?: White colony on a Lac Mac + Amp Plate: A. Has MuD1 in a gene that’s turned on. B. Has MuD1 in a gene that is turned off. C. Has MuD1 in a gene that may be on or off. If gene off, can’t make lacZ. When p is on, it gives you red; but when lacZ goes to the left and p,on to the left, would give you white. Therefore, MuD1 could be on or off. When you want to count gametes, what type of cross do you do? Test cross. But we did an F1 x F1 cross! Only look at males and ignore female cross. Recripocal pairs: grouped from homologous pairs put together In the example shown in lecture: The difference between e and g is 49.3. We only saw a crossover distance of 39.7. Why? You can’t see double crossovers. Double crossovers don’t get counted. Reduction of straight probability; coincidence....
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