Sarah Pham (slp2723) Section #48955 BIO 206L: Exercise 3 Lab Report 1. a) The oculars are located at the top of the microscope above the objective lenses. Oculars function as eyepieces which produces a magnified virtual image of the real image. b) The objective lenses are located above the stage and below the oculars. Objective lenses function in collecting the light after it passes through the object to produce a magnified real image. c) The stage is located right below the objective lenses and above the condenser. The stage functions as a moveable platform that moves the specimen closer or further from the objective lenses. d) The condenser is located beneath the stage and contains lenses which shape the direction of the light before it encounters the object. 2. Limitations to clearly viewing living cells using light microscopy is that with a light microscope you can only view objects that are as small as the shortest wavelength of visible light; any microorganism that is smaller than that cannot be seen by the light microscope. The maximum resolution of a light microscope is 250nm which means that you cannot distinguish two objects that are closer than 250nm apart. Moreover, microscopic objects have no contrast under a light microscope which means that most specimens are completely transparent. In order to see contrast in the specimen, dyes such as stains are used. However, most stains tend
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