practice exam 2 answer key

practice exam 2 answer key - H" Last Name {print}...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
Background image of page 5
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: H" Last Name {print} war 3 Cit] First Name {print} é%fi 32 I: —__, Tats ,__ ___,__—_,_ Lah Section {time and day} BISC 32d Midterm Exam 2 -- lWZZIflfil Total Score (out of 100 pts} _ Multiple Choice {3 pts. Each} Choose the BEST answer! 1] 1|ul'v'hy is cohesion necessary for proper chromosome segregation during mitosis“? a for genetic exchange {crossing over) é to ensure the two sister chromatids are segregated into different daughter cells c} to ensure sister ehromatids are segregated together into the same daughter cell d} to make sure homologous chromosomes segregate into different daughter cells 2} DNA sequencing requires: a} DNA polymerase b} Dideoxynocleotides c] Deoxynucleotides @ All of the above 3} In a haploid ceil. which of the following does not occur: at} mitosis b} homologous recombination (? meiosis } cohesion 4} When comparing the genomes of different organisms, gene density is found to: a} increase with increasing complexity of the organism @ decrease with increasing complexity of the organism c) is essentially constant among all organisms d} it varies. but there is no clear relationship to the complexity of the organism 5} 1‘J‘v’hieh of the following is not involved in processing of Ukazaiti fragments: a} DNA polymerization b} DNA ligation c} Endonuclease or exonuclease (g) RNA polymerization b] In prokaryotic cells, detection of hemi-rnethylated DNA is required by which of the following processes: a] homologous recombination @ mismatch repair c} photoreactivation of thymine dimmers d} base excision repair 7"} Making a specific gene deletion in any organism requires: a} transposon mutagenesis b) knowledge oi" the gene‘s function c a haploid cell d homologous recombination . art Name [print] .__ .. . _ . First Name {print} 8) A diploid yeast cell has a normal anti a mutant allele of a gene involved in biosyntliesis of histidine. A haploid yeast cell with the mutant allele ofthis gene is unable to grow without histidine added to its growth medium; however, the diploid cell described above is m to grow without added liistidine. The ability of the diploid cell to grow without add histidinc is clue to: § Complementation .] DNA repair cl Homologous recombination d] All of the above 9] to pts.) During DNA replication, DNA polymerase incorporates a base incorrectly into the nascent DNA. Nani; the main mechanism that will act to correct the error. Then assuming the first mechanism fails. name the second mechanism that will act to correct the error. He sure to indicate which is lirst and second. l) Prac’wcjlf‘j (:7)! "iv ‘5" E‘Fflan(Fafl) 2—1 MSW-I54!" Walt” ill] I[ pts.) Name or briefly describe: a} the enzymatic activity of telomerase that allows it to extend the tclotneres rersc'se ltfinserifiwse bl which DN A strand is extended by telomerase? Blew e] the nature of the telomerie DNA sequence that is necessary for telomerase to act i+ 15 rage-Hid we d] the physical component of te omerase that allows it to bind telotneres specifically KNA. ll} {12 pts.) From each of the following questions. choose the BEST answer from the following list of enzymatic activities. Base your answer on the enzymatic activin at the DNAIRNA level (he. the specific chemical reaction that is carried out} List to choose from: DNA glycosylase, DNA polymerase, RNA polymerase, DNA ligase, Type I DNA topoisoprnerase, Type II DNA 'l‘opoisomerase, DNA recotnbittase, DNA endonuclease. 5‘ to 3‘ DNA eaonuclease, 3‘ to 5‘ DNA exonuclease, DNA helicase, DNA Primase a} Which activity is the opposite of DNA polymerase?r 3' 5h; '5’ € Mnuflflfl bl Which activity is the opposite of DNA endonuclease'r‘ 9“ p.- [‘,j: 5.: c] Which shares similar acrivities as Type II DNAE'l'opoisomerase‘? Fore mlpmaw I _ . . . r - ‘9 dl ill. inch is similar to DNA Primasc. w Pr RAVI/r5425"! t1} tName {print} ___ First Name {print} 12} {IE pts.) Draw the structure of a DNPi ieplication fork showing template and nascent strands. Use lines for each DNA strand and indicate their orientations {5‘ or 3‘}. Also use arrowheads on the ends of nascent strand to indicate their direction of synthesis. Indicate the leading and taggin g strands and show at least two Dkazalti fragments on the lagging strand. Indicate which Ditaraki Fragment was synthesized first. and which was second};' how RNA primers. Do not try to show proteins. 13} (It) pts.) a} Describe the basic eruclure ot'a nucleosonte in detail tnatne proteins, how many. how much DNA}. 34?" 59 Eli'- 5re Lat—offal "BM; gaflflfi a far-q Wt“ partieQJp-filarck (Wt-er 2L twain as? 1}“;pr 42A; H25, H. of Z {flat-#33 Ala-aw 0E” 35 fiww His») Li H F t h] Deserihe two additional hierarchies of chromatin structure. In each case. name andfor briefly: describe the basic slrnctnretorganizgtion of the DNAtchn'nnatin and nanregnj. groteins or Jrotein structures intro] 1red. ;) 30 {W'- ‘P'ltm'l‘ 3'5 a man! Comoflowaed 5mm flaws finc'iécfiofi! tr) EFF—_- Mggrdfiucleojm 3? was My stem at bran/xi... am now cejicm swam Eflffim‘i PHI? 4 '21:)" 0f kailtcgf (wgfw-‘a'flw‘l‘ meet 3) EifircWF-flt'i'tn {pulp-I 0r Admitth it (grit, {1?wa DNA irra- m bore" 3‘” ‘ é W 42: «form (was eti— alarm-who grew-a a (WWI axis. 3) cmdanain PE rtnjfiéflpfififl‘gffl‘ieifi int A‘gfiaflj Swansea first w”. New m 1m 11'fllll'l.‘ tPl. Illl} I. LI. al- J. 'lflllI'Lr 1P1 lIl'I-J 14} {it} pta.) Draw the structure of a Hollitlay Junction ootwoon tho following two homologous DNA molooulos. Make sure you indicate 5’ and 3*. The arrows: intiioato the site of single—strand nicks that initiato tho Hollitiay Junction. Make a aooond drawing after migration of tho Ilollioa}r Junction to givo a holoroduploa at region B. Finally, uso arrows on your second drawing to indicate the poaition of cleavage. to resolve tho liotiidayjonotion without a crossovor. A B D A B D a h at a b ti U19"! ast Name {print} _ _ __ First Name [priltt]_ 15} {12 pts.) List the basic reegentsfmelecnles needed to carry [1:11 a PCR reaction. and briefly describe the three basic steps in the PCR reactinn ilseff pus prisms: wig-gem) 3W9: 'ILWIIJ'H primfif E1¥emsrm r FfiIR W +0 ‘737— 4-; saw pfizynfir—c'fiw 71% {FW Yam 1(1) {:3 cits.)I Show the structure ni'the rneieculets] that result frnin a singie [TF03501-‘Ci' between the henmingnus regiens, indicated by arrows. Indicate the genetic markers (A. B. [7. and D] en the resulting meleeuielfis]. A — 4.- 3 (3 —I- T} MM ...
View Full Document

Page1 / 5

practice exam 2 answer key - H" Last Name {print}...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online