Lab 18 Buffers (1) - Lab 18 Buffer Lab In this experiment...

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Lab 18: Buffer LabIn this experiment you will make a buffer that contains a weak acid and its conjugate base. Then you will putstress on the system by adding a base (or acid) of known concentration and determine the pH after each additionof base (or acid). Finally, using the Henderson-Hasslebach equation, you will calculate the theoretical pH anddetermine the % error for your experimental measurements.HA H++ A-pH = pKa+ log ( [A-] / [HA] )Part I:PROCEDUREA) pH Probe Calibration1.Press the On/Offbutton to turn on the power to the MeasureNet Workstation.2.Press Main Menu, then press F3(pH vs. mVolts), then press F1(pH vs. Time).3.Press Calibrate. Rinse and dry the pH probe. Place the pH probe in the container of pH 7.00 buffer solution. Using a thermometer, determine the temperature of the pH 7.00 buffer solution and enter it at the workstation. Press Enter.4.Enter 7.00 as the pH of the buffer solution at the workstation, press Enter. 5.Gently stir the buffer solution with a stirring rod. When the displayed pH value stabilizes, press Enter.

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