Lecture 7 - 3-10-08 Cloning

Lecture 7 - 3-10-08 Cloning - Genetic Engineering - Cloning...

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Genetic Engineering - Cloning Genetic Engineering - Cloning March 10, 2008 March 10, 2008
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Notes and Reminders Strep Lab Report 2 Due this week in lab! Midterm will be 3/31 7:00p – 9:00p, LH2 Alternate exam time - 10:00 am, BS008 Final will be May 12, 4:00p – 6:00p, LH2. Show up late ≈ missing a quiz!
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This Week We will be analyzing the results of the end of a cloning procedure Cloning – Taking a gene from one organism and inserting it into another We copied the pho A gene from one strain of E. coli and inserted in into another strain
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Nucleases – enzymes that cut DNA Exonucleases – cut DNA from the ends and work inward Endonucleases – cut DNA in the inside of the molecule Both cut randomly, just anywhere, which is not very useful. But a type of endonuclease called a restriction enzyme is more specific where it cuts!
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Restriction Enzymes Restriction Enzymes Eco RI – Comes from E. coli , 1 st one discovered 5’- G A A T T C 3’- C T T A A G Cuts Cuts A A T T C G G C T T A A “Sticky Ends”
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Restriction Enzymes Restriction enzymes found in bacteria, and named for the bacterium they were found in. Most restriction enzymes recognize and cut a 4 to 10 base pair long palindromic sequence. A
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Lecture 7 - 3-10-08 Cloning - Genetic Engineering - Cloning...

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