Lecture 9 - Lecture 8 - Anion Chromatography, Suppressed...

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Anal Chem 251 Lect 8 Anion Chromatography 1 Lecture 8 - Anion Chromatography, Suppressed Ion Chromatography Chapter 23 : Problems 23:1,4,8 Corresponding Lab: Ion Chromatography – Anions in Seawater
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Anal Chem 251 Lect 8 Anion Chromatography 2 Measurement of Ionic Solids in Seawater The first experiment you performed involved dissolved substances in filtered seawater. Aqueous solutions of ionic solids conduct electricity, the more ions, the greater the conductivity. In a later experiment, you will also measure the salinity of a sample of water with a salinity meter by measuring the electrical conductivity of the water. However, this won’t give any information on the nature of the ions.
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Anal Chem 251 Lect 8 Anion Chromatography 3 Anions in Seawater As you know, there are different ions in seawater. You can refer to LEO-15 prelab. The anions are: Chloride Ion : high concentration due to salting of the roads Sulfate Ion : from acid water Nitrate/phosphate Ions : run off water from lawn and agricultural land that are treated with fertilizer. High nitrate/phosphate cause algae to grow and when they die in late summer, it contributes to eutrophication. Eutrophication occurs when large concentrations of organic matter in bodies of water react with all the available oxygen, leaving little or nothing for the fish to breathe. High concentration causes algae to grow and when they die late summer.
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Anal Chem 251 Lect 8 Anion Chromatography 4 Simultaneous Analysis of Anions and Cations • Traditionally, these ions were analyzed individually, that means they were separated and treated one by one such as the analysis of chloride ion and sulfate ion that you performed in the gravimetry experiment • With Ion Chromatography, we can analyze all anions as a group and all cations together. It is rapid and very convenient. Then we can compare the results to the other techniques that we have used.
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Anal Chem 251 Lect 8 Anion Chromatography 5 Review of Chromatography The development of chromatography as an analytical tool began in 1903 when Mikail S. Tsweet, a Russian botanist, discovered that he could separate colored leaf pigments by passing a solution through a column packed with absorbent chalk particles. Tsweet named this new method chromatography (chroma = color, graphein” means to write ) Since its introduction in the mid 1970’s, Ion Chromatography (IC) has become widely used as an analytical technique for the determination of polar and ionic species.
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Anal Chem 251 Lect 8 Anion Chromatography 6 Review of Chromatography (Continued) • Chromatography is defined as the separation of components in a mixture by partitioning between a mobile phase and a stationary phase. The mobile phase is the eluent and the stationary phase is the column.
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Lecture 9 - Lecture 8 - Anion Chromatography, Suppressed...

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