Biol 111 Lab Final Review

Biol 111 Lab Final Review - Exercise 7 Cellular Metabolism...

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Exercise 7: Cellular Metabolism Oxidation of glucose The two key stages in the oxidation of glucose are Glycolysis and the Krebs, or citric acid, Cycle. Glycolysis is an anaerobic process that occurs in the absence of oxygen. It takes place in the Cytoplasm of cells Glycolysis yields 2 ATP molecules and in the absence of oxygen may constitute the primary source of ATP synthesis. It results in the production of pyruvate (pyruvic acid). Pyruvate is further metabolized by another series of anaerobic reactions called fermentation. In organisms such as yeasts, alcoholic fermentation results in the production of alcohol (ethanol) and carbon dioxide. In animal cells and humans, lactic acid fermentation results in the production of lactate (lactic acid) Anaerobic Respiration It is possible to observe CO2 production in yeasts by using an apparatus that sends the gas through a pH indicator solution. The acid-base indicator bromothymol blue is used. Bromothymol blue forms carbolic acid in the presence of CO2 which causes the pH to become more acidic and change the color of the solution. As carbon dioxide passes from the flask through the U-tube into the cylinder, the bromothymol blue solution changes color as carbolic acid is produced. Aerobic Respiration The Krebs Cycle Hyrdogen atoms are transferred from succinate to the electron carrier coenzyme FAD. In this experiment you will substitue 2,6- dichloroindophenol (DPIP) for FAD. When DPIP is chemically reduced, it changes color from
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Biol 111 Lab Final Review - Exercise 7 Cellular Metabolism...

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