test 2 review - 9 10 11 12 13 13 18 20 25 27 Bacterial...

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9 13 Bacterial Growth 6 6 10 18 Bacterial Genetics 8,9 8,9 11 20 Bacterial Genetics 8,9 8,9 12 25 Virology – Bacterial Viruses 13 13 13 27 Virology – Animal Viruses 13 21 (p 599-605) 22 (p 625-635) 23 (p 657-660) 24 (p 691-694) 13 21 (p 613-629) 22 (p 643-659) 23 (p 675-691) 24 (p 713-733) 14 Mar 3 Intermediate Organisms 11 (p 306; 324; 326- 328) 23 (p 655-657) 24 (p 688-689; 690) 11 (p 316-317; 332; 336-338) 23 (p 687-690) 24 (p 726-729) Bacterial Growth: Two categories: physical and chemical Physical - temperature: o psychrophiles (cold loving microbes) 0-20 degrees Celsius optimal=15 o mesophiles (moderate temp loving) 25-40 o= 37= our body temp o thermophiles (heat loving) 45-100 o=60 o hyperthermophiles (so hot) 60-110 o=100…found in ocean and geisers. - pH o bacteria grow best near neutrality 6.5-7.5 o buffer- solution that helps pH not drop or increase to maintain pH o H+ + anion= acid o OH + cation = base o H+ + OH- = water o pH= - log (10?) (H+) concentration o pH+pOH=14 o H+ * OH- = 10 to the negative 7 o pH= pOH= 7 o molecular concentration= Moles/Liter o water pH= -log (10 to the negative 7)= -(-7)= 7= neutral o blood= 7, stomach acid= 1-2, household bleach=12-14 - Osmotic pressure o Hypertonic has more concentration leading to less water concentration outside the cells o Hypotonic: has less concentration leading to more water concentration outside cells. o Halophiles require salt concentration for growth. Chemical
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Chemical requirements are Carbon, Nitrogen, Sulfur, and phosphorus. Nitrogen fixation is when some cells take N2 directly from the atmosphere for ammonia. 5 maj. Groups of Oxygen bacterial growth: 1. obligate aerobic: requires oxygen!: Pseudomonas aeruginosa 2. microaerophilic: requires oxygen but at lower concentration than the atmosphere: Neisseria gonorrheae. 3. facultative anaerobic: better without O2: Escherichia coli 4. areotolerant anaerobic: equally low with or without O2 doesn’t use it but doesn’t cease growth: look up 5. obligate anaerobic: cannot grow in O2: Clostridium tetani or any clostridium Binary fission: bacteria reproduction; doubling cells - 25-30 min. generation time in rich media. - More carbon source the faster - Full cycle takes about 60 min. - Generation time calculation: o N=No2 to the n o N=number of bacteria after certain number of generations o No=initial number of bacteria o n=total number of generations which equals time in hours divided by generation time=(hours/gener) Problem 100 bacteria 6 hours 2 hours/generation 6/2= 3= n 100* 2 to the third = 800
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Growth Curve: log keeps numbers down. 1.
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This note was uploaded on 04/28/2008 for the course BIO 226N taught by Professor Blinkova during the Spring '08 term at University of Texas.

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test 2 review - 9 10 11 12 13 13 18 20 25 27 Bacterial...

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