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Gargar,Keya_MT-2A_Lab_Act_4_Macroscopic and_Chemical_Examination_of_Urine.pdf

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COLEGIO SAN AGUSTIN-BACOLODCollege of Health and Allied ProfessionsMLS 108 LABORATORY ACTIVITY SHEETMedical Technology ProgramLaboratory Exercise # 4Macroscopic and Chemical Examination of FecesIntroduction:Fecal matter is not only examined microscopically but as well as macroscopically and chemicallybecause these examinations can also be of help in diagnosing a disease.Objectives:After performing this exercise, the student should be able to:1.macroscopically describe the appearance of a normal and abnormal stool; and2.do or perform and interpret chemical tests for stool samples.Materials:8% H2O2Stool specimenNitrazine PaperBenzidine solutionHema - screen kit10% Barium chloride aqueous solutionProcedure:I.Macroscopic Examination1.Prepare the start of tests, clean the working area and cover it with newspaper enough tohold your specimen.2.Open the container of stool sample aseptically to avoid contaminating hands.Place coverof container on the covered area side up.3.Observe color, odor, form and consistency of stool sample (stab the sample with anapplicator stick).4.Note the presence of adult parasite or proglotidds, undigested food, fibers, concretions,mucus, blood streak and fats.5.Specimen may be liquefied with distilled water and strained through a clean wire meshsieve or nylon sieve (wire or nylon screen) to recover smaller worms and scolices.II.Chemical ExaminationsA.pH (Nitrazine Paper test)Procedure:1.With an applicator stick, smear a small amount of fecal emulsion on piece of nitrazinepaper.2.Wait for two minutes then observe color change and compare with color chart.
COLEGIO SAN AGUSTIN-BACOLODCollege of Health and Allied ProfessionsMLS 108 LABORATORY ACTIVITY SHEETMedical Technology ProgramB.Occult Blooda)Benzidine TestPrinciple:The peroxidases contained in the heme portion of the blood react with H2O2liberatingoxygen which in turn oxidizes benzidine to form a blue colored compound.Procedure:1.With an applicator stick, smear a small amount of stool on a clean filter paper.2.Add 2 drops of 8% H2O2on the smear and then add also two drops of benzidine solution.3.Let stand for about 30-60 seconds and note the color change.Interpretation:No color change- NegativeGreenish linge-TraceLight green-1+Dark green-2+Blue green-3+Deep blue-4+b)Hema-screenPrinciple:When stool specimens containing occult blood are applied to hema-screen test paper, thehemoglobin portion of the occult blood comes in contact with the guaiac. When the hema-screenperoxide developing solution is added, a guaiac-peroxidase like reaction occurs. The chemicalreaction becomes visible by the appearance of a blue-green color between 30 seconds if occultblood is present.Procedure:1.With an applicator, apply very thin smear of stool the area indicated with roman numeral I.

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Feces, COLEGIO SAN AGUSTIN BACOLOD, College of Health and Allied Professions

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