LS1a Lab5 NoorBeckwith

LS1a Lab5 NoorBeckwith - Performed on 10.X.2007 Noor...

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Performed on 10.X.2007 Noor Beckwith Partners: Chioma Madubata and Mark Pacult LS1a – Sec. W6-9 CA: Koh Lab 5: “Finding the needle in the haystack” Responses 1 1. Attach a picture of your gel and answer the following: a. How many protein bands appeared in each of your lab group’s lanes (left side 1 )? C – there are >12 relatively distinct bands FT – also >12 W1 – ~9 W2 – 0 E1 – 3 E2 – 3 (1 dominant band, 2 extremely faint bands) b. What is the size of the dominant band in the eluant lanes (E1 and E2)? ~39-40 kDa c. Is the intensity of that band greater or less than the intensity of the corresponding size in the crude lysate? The flow-through lane? The wash lanes? Greater in all cases 2. Why does the number of protein bands decrease in each of the lanes compared to the crude lysate lane? Each step of the procedure is meant to wash out non-His-tagged proteins that have non-specifically become stuck in the column. Thus, as is desired, each successive wash yields fewer bands/bands of diminishing crispness
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LS1a Lab5 NoorBeckwith - Performed on 10.X.2007 Noor...

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