CHE 558 Experiment 13
Biochemistry Lab
Crystallization of Lysozyme
Objectives
1. To gain experience in crystallization techniques
2. To see if your purified lysozyme crystallizes
Experimental Procedures
Materials
Purified lysozyme from the following week’s experiment
25 mg/mL lysozyme solution
1 M sodium acetate, pH 4.7
10% sodium chloride
forcep
scissor
plastic microscope slides
24 well plate
syringe with vacuum grease
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Methods:
Part A: Preparing the 24 well plate
1. Place two kimwipes next to each other and then place the 24 well plate on it.
2. On the lid, label the wells.
The first row is A, the next is B, then C, and then D.
The columns are numbered numerically from left to right starting with 1 and
ending in 6.
3. Grease the overhang of each well of the 24 well plate. (Hint: just like icing the
edges of a cake)
4. Fill each well using the chart below.
(Hint: first put sodium acetate in all the
wells then the sodium chloride, then the water.
This will help prevent changing
pipette tips)
Position
1 M sodium acetate
(
μ

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- Spring '07
- Mazlo
- Chemistry, Sodium chloride, Crystal, Microscope slide, Pipette, Crystallization
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