Available online a t Pelagia Research Library European Journal of Experimental Biology, 2011, 1 (2):118-124 ISSN: 2248 –9215 118 Pelagia Research Library Isolation, Identification and Characterization of Escherichia Coli from Urine Samples and their Antibiotic Sensitivity Pattern Manaal Zahera*, Chetan Rastogi, Pushpendra Singh, Sana Iram, Shumaila Khalid and Akhilesh Kushwaha Institute of Transgene Life Sciences, C-4/1, Vikalp Khand, Gomtinagar, Chinhut, Lucknow, U.P ______________________________________________________________________________ ABSTRACT A urinary tract infection is an infection that begins in your urinary system. Urinary system is composed of the kidneys, ureters, bladder and urethra. Though Escherichia Coli, which is normally present in the gastrointestinal tract is the commonest causative organism, other gram negative colonic bacteria. Any part of your urinary system can become infected, but most infections involve the lower urinary tract, the bladder and the urethra. Women are at greater risk of developing a urinary tract infection than are men. A urinary tract infection limited to your bladder can be painful and annoying. However, serious consequences can occur if a urinary tract infection spreads to your kidneys. Antibiotics are the typical treatment for a urinary tract infection like (Norfloxacin, Tetracycline, and Ampicillin etc) .By Antibiotic sensitivity pattern we can measure the Zone of diameter in millimeters. The size of zone of inhibition was interrupted by referring to the CLSI (Clinical Laboratory Standard Institute) guidelines and organism was labeled susceptible, intermediate, or resistance accordingly. Key words: Urinary Tract Infection, Gram negative Rod shaped, Escherichia species Antibiotic sensitivity pattern, Norfloxacin, Tetracycline, Ampicillin, Zone diameters in millimeters. ______________________________________________________________________________ INTRODUCTION A urinary tract infection (UTI) is a bacterial infection that affects any part of the urinary tract. Symptoms include frequent feeling and or need to urinate, pain during urination, and cloudy urine (Urinary Tract Infections 2010).
Manaal Zahera et al Euro. J. Exp. Bio., 2011, 1(2):118-124 ______________________________________________________________________________ 119 Pelagia Research Library The main causal agent is Escherichia coli . Although urine contains a variety of fluids, salts, and waste products, it does not usually have bacteria in it (Adult Health Advisor 2005). When bacteria get into the bladder or kidney and multiply in the urine, they may cause a UTI. The most common type of UTI is acute cystitis often referred to as a bladder infection. An infection of the upper urinary tract or kidney is known as pyelonephritis, and is potentially more serious. Although they cause discomfort, urinary tract infections can usually be easily treated with a short course of antibiotics with no significant difference between the classes of antibiotics commonly used (Zalmanovici Trestioreanu A, Green H et al : 2010).
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