isolation-identification-and-antimicrobial-susceptibility-pattern-analysis-of-escherichia-coli-isola - Isolation identification and antimicrobial

Isolation-identification-and-antimicrobial-susceptibility-pattern-analysis-of-escherichia-coli-isola

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© Asian Journal of Biomedical and Pharmaceutical Sciences, 2016. Isolation, identification and antimicrobial susceptibility pattern analysis of Escherichia coli isolated from clinical samples of Bangladesh Urinary tract infection (UTI) is one of the most common infections of the world caused by mainly Escherichia coli . The purpose of this study was to identify E. coli as causative agent of UTI in patient of different age groups and to investigate their responses against commonly used antibiotics. Altogether, 480 urine samples were analyzed by culture method. The sam- ples were equally streaked on Blood agar, MacConkey, and Eosin Methylene Blue Agar and then incubated at 37 o C for 24 hours. After 24 hours of incubation, E. coli was identified on the basis of morphological characteristics of colony on culture media. For further confir- mation of the presence of E. coli , Gram staining and conventional biochemical tests were also performed. Disk diffusion method was used for susceptibility testing against seventeen different antibiotics on Muller Hinton agar. Among the 480 urine samples, 81 samples were positive for E. coli . It was found that the females were more prone to UTI than males. The result of antibiotic sensitivity test on E. coli isolates demonstrated that they were highly sensitive to Amikacin, Gentamycin, Netilmycin, Imipenem, Meropenem, Pipracillin-Ta- zobactam, Tobramycin, Nitrofurantoin , Azithromycin, Levofloxacin, and Ciprofloxacin. E. coli was found intermediate sensitive to third-generation Cephalosporins such as Cefixime, Cefotaxime, Ceftazidime, Ceftriaxone and least sensitive to Cotrimoxazole and Nalidixic acid. Thus, all antibiotics used in present study except Cotrimoxazole and Nalidixic acid, could be the choice for empirical treatment of UTI. Keywords: Urinary tract infection, Escherichia coli , antibiotics, antimicrobial resistance. ABSTRACT : E. coli is an important normal flora of human and other mammals. It is widely distributed in the environment and also causes a variety of infection including urinary tract infection. Urinary tract infection (UTI) is the most com- mon infection of human population. UTI is defined as the persistence of actively growing microorganisms within the urinary tract. Microorganisms causing UTI almost come from the skin at or near the opening of the urethra. In case of UTI, most susceptible groups are neonates, girls, young women and men. In case of adult person, it occurs more commonly in women than men because the female ure- thra is much shorter and closer to anus, therefore up to 40% women develop UTI at least once during their lives and a significant numbers of these women suffer recurrent UTI [1]. On the other hand, the secretion of male prostate contains the bactericide substances and Zn which play a vital role in countering with E. coli and prevent this kind of infection in men. Although antibiotics are widely avail- able, UTI still remains one of the most common clinical complications. Because now a day’s antibiotic resistance
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  • Fall '13
  • Omair Gul
  • Escherichia coli, urinary tract infection, urinary tract

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