gupta2015 - Planta DOI 10.1007\/s00425-014-2239-3 REVIEW Insights into structural and functional diversity of Dof(DNA binding with one finger

gupta2015 - Planta DOI 10.1007/s00425-014-2239-3 REVIEW...

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REVIEW Insights into structural and functional diversity of Dof (DNA binding with one finger) transcription factor S. Gupta N. Malviya H. Kushwaha J. Nasim N. C. Bisht V. K. Singh D. Yadav Received: 21 August 2014 / Accepted: 25 December 2014 Ó Springer-Verlag Berlin Heidelberg 2015 Abstract Main conclusion The structural, functional and in- silico studies of Dof transcription factor attempted so far reveals immense opportunity to analyze the plant genomes in terms of number of Dof genes and discuss in light of the evolution. The multiple functions of Dof genes needs to explored for crop improvement. Transcription factors play a very vital role in gene regu- lation at transcriptional level and are being extensively studied across phylas. In recent years, sequencing of plant genomes has led to genome-wide identification and char- acterizations of diverse types of plant-specific transcription factor gene family providing key insights into their struc- tural and functional diversity. The DNA binding with one finger (Dof), a class belonging to C2H2-type zinc finger family proteins, is a plant-specific transcription factor having multiple roles such as seed maturation and germi- nation, phytohormone and light-mediated regulation and plant responses to biotic and abiotic stresses. Dof proteins are present across plant lineage, from green algae to higher angiosperm, and represent a unique class of transcription factor having bifunctional binding activities, with both DNA and proteins, to regulate the complex transcriptional machinery in plant cells. The structural and functional diversity of the Dof transcription factor family along with the bioinformatics analysis highlighting the phylogeny of Dof families is reviewed in light of its importance in plant biotechnology for crop improvement. Keywords Transcription factor Á Dof Á Zinc finger Á Gene regulation Á In silico Introduction Transcription factors (TFs) recognize specific DNA sequence elements within promoter and are responsible for activating or repressing the activity of RNA polymerase vis- a-vis controlling the temporal and spatial expression of a target gene. TFs manifest their function through direct or indirect (through co-activators) interactions with the basal apparatus that lead to changes in the properties of RNA polymerase. A typical plant transcription factor contains a DNA-binding domain, an oligomerization site, a transcrip- tion regulation domain and a nuclear localization signal. Most of the transcription factors have only one type of DNA- binding region and oligomerization region although some lack transcription regulation domain or a specific DNA- binding region. The DNA - binding domain of a TF interacts with DNA at the cis - sequence elements owing to the Electronic supplementary material The online version of this article (doi: 10.1007/s00425-014-2239-3 ) contains supplementary material, which is available to authorized users.
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